![]() Cyclic AMP and phorbol esters separately induce growth inhibition, calcitonin secretion, and calcitonin gene transcription in cultured human medullary thyroid carcinoma. Google Scholar deBustros A, Baylin SB, Levine MA, Nelkin BD. Most sensitive (0.006 pmol/ml and 0.027 pmol/ml) and complete colorimetric kit for intra- and extracellular cAMP quantification. A cyclic AMP- and phorbol ester-inducible DNA element. Most sensitive (0.027 pmol/ml) colorimetric kit for extracellular cAMP quantification. Most sensitive (0.006 pmol/ml) colorimetric kit for intracellular cAMP quantification. This product is compatible with the Absorbance 96 Plate Reader.ĮLISA, Colorimetric detection | Print as PDF cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. For the cyclic AMP assay y-PATP (10 u M) is incubated with 10 mM magnesium acetate. GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer 2, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, TriethylamineĬyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. PROTEIN KINASE ASSAY The method is based upon the ability of low. Store all components at +4☌, except standard and conjugate at -20☌. Cited sample types include cell lysate, tissue, and urine.ĪMP, ATP, cyclic GMP, GMP, GTP, cyclic UMP, CTP (<0.001%) Non-acetylated: 0.49 pmol/ml (range 0.78 - 200 pmol/ml)Īcetylated: 0.027 pmol/ml (range 0.078 - 20 pmol/ml)įor the quantitative determination of cAMP in culture supernatants, serum, and saliva from any species.
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